diff --git a/crates/uv-resolver/src/lock.rs b/crates/uv-resolver/src/lock.rs
index 078db6050..f2c3e5dfe 100644
--- a/crates/uv-resolver/src/lock.rs
+++ b/crates/uv-resolver/src/lock.rs
@@ -20,7 +20,7 @@ use distribution_types::{
     GitSourceDist, IndexUrl, PathBuiltDist, PathSourceDist, RegistryBuiltDist, RegistryBuiltWheel,
     RegistrySourceDist, RemoteSource, Resolution, ResolvedDist, ToUrlError,
 };
-use pep440_rs::Version;
+use pep440_rs::{Version, VersionSpecifiers};
 use pep508_rs::{MarkerEnvironment, MarkerTree, VerbatimUrl};
 use platform_tags::{TagCompatibility, TagPriority, Tags};
 use pypi_types::{HashDigest, ParsedArchiveUrl, ParsedGitUrl};
@@ -36,6 +36,8 @@ use crate::{lock, ResolutionGraph};
 pub struct Lock {
     version: u32,
     distributions: Vec<Distribution>,
+    /// The range of supported Python versions.
+    requires_python: Option<VersionSpecifiers>,
     /// A map from distribution ID to index in `distributions`.
     ///
     /// This can be used to quickly lookup the full distribution for any ID
@@ -87,15 +89,21 @@ impl Lock {
             }
         }
 
-        let lock = Self::new(locked_dists.into_values().collect())?;
+        let distributions = locked_dists.into_values().collect();
+        let requires_python = graph.requires_python.clone();
+        let lock = Self::new(distributions, requires_python)?;
         Ok(lock)
     }
 
     /// Initialize a [`Lock`] from a list of [`Distribution`] entries.
-    fn new(distributions: Vec<Distribution>) -> Result<Self, LockError> {
+    fn new(
+        distributions: Vec<Distribution>,
+        requires_python: Option<VersionSpecifiers>,
+    ) -> Result<Self, LockError> {
         let wire = LockWire {
             version: 1,
             distributions,
+            requires_python,
         };
         Self::try_from(wire)
     }
@@ -196,6 +204,8 @@ struct LockWire {
     version: u32,
     #[serde(rename = "distribution")]
     distributions: Vec<Distribution>,
+    #[serde(rename = "requires-python")]
+    requires_python: Option<VersionSpecifiers>,
 }
 
 impl From<Lock> for LockWire {
@@ -203,6 +213,7 @@ impl From<Lock> for LockWire {
         LockWire {
             version: lock.version,
             distributions: lock.distributions,
+            requires_python: lock.requires_python,
         }
     }
 }
@@ -215,6 +226,10 @@ impl Lock {
         let mut doc = toml_edit::DocumentMut::new();
         doc.insert("version", value(i64::from(self.version)));
 
+        if let Some(ref requires_python) = self.requires_python {
+            doc.insert("requires-python", value(requires_python.to_string()));
+        }
+
         let mut distributions = ArrayOfTables::new();
         for dist in &self.distributions {
             let mut table = Table::new();
@@ -344,6 +359,7 @@ impl TryFrom<LockWire> for Lock {
         Ok(Lock {
             version: wire.version,
             distributions: wire.distributions,
+            requires_python: wire.requires_python,
             by_id,
         })
     }
diff --git a/crates/uv-resolver/src/python_requirement.rs b/crates/uv-resolver/src/python_requirement.rs
index 0b8c2598e..f1c4278ab 100644
--- a/crates/uv-resolver/src/python_requirement.rs
+++ b/crates/uv-resolver/src/python_requirement.rs
@@ -60,13 +60,14 @@ impl PythonRequirement {
 
 #[derive(Debug, Clone, Eq, PartialEq)]
 pub enum RequiresPython {
-    /// The `RequiresPython` specifier is a single version specifier, as provided via
+    /// The [`RequiresPython`] specifier is a single version specifier, as provided via
     /// `--python-version` on the command line.
     ///
     /// The use of a separate enum variant allows us to use a verbatim representation when reporting
     /// back to the user.
     Specifier(StringVersion),
-    /// The `RequiresPython` specifier is a set of version specifiers.
+    /// The [`RequiresPython`] specifier is a set of version specifiers, as extracted from the
+    /// `Requires-Python` field in a `pyproject.toml` or `METADATA` file.
     Specifiers(VersionSpecifiers),
 }
 
@@ -93,6 +94,14 @@ impl RequiresPython {
             }
         }
     }
+
+    /// Returns the [`VersionSpecifiers`] for the [`RequiresPython`] specifier.
+    pub fn as_specifiers(&self) -> Option<&VersionSpecifiers> {
+        match self {
+            RequiresPython::Specifier(_) => None,
+            RequiresPython::Specifiers(specifiers) => Some(specifiers),
+        }
+    }
 }
 
 impl std::fmt::Display for RequiresPython {
diff --git a/crates/uv-resolver/src/resolution/graph.rs b/crates/uv-resolver/src/resolution/graph.rs
index b63e4626b..6a6a91389 100644
--- a/crates/uv-resolver/src/resolution/graph.rs
+++ b/crates/uv-resolver/src/resolution/graph.rs
@@ -9,7 +9,7 @@ use rustc_hash::{FxHashMap, FxHashSet};
 use distribution_types::{
     Dist, DistributionMetadata, Name, ResolutionDiagnostic, VersionId, VersionOrUrlRef,
 };
-use pep440_rs::{Version, VersionSpecifier};
+use pep440_rs::{Version, VersionSpecifier, VersionSpecifiers};
 use pep508_rs::{MarkerEnvironment, MarkerTree};
 use pypi_types::{ParsedUrlError, Yanked};
 use uv_git::GitResolver;
@@ -17,10 +17,13 @@ use uv_normalize::{ExtraName, PackageName};
 
 use crate::preferences::Preferences;
 use crate::pubgrub::{PubGrubDistribution, PubGrubPackageInner};
+use crate::python_requirement::RequiresPython;
 use crate::redirect::url_to_precise;
 use crate::resolution::AnnotatedDist;
 use crate::resolver::Resolution;
-use crate::{InMemoryIndex, Manifest, MetadataResponse, ResolveError, VersionsResponse};
+use crate::{
+    InMemoryIndex, Manifest, MetadataResponse, PythonRequirement, ResolveError, VersionsResponse,
+};
 
 /// A complete resolution graph in which every node represents a pinned package and every edge
 /// represents a dependency between two pinned packages.
@@ -28,6 +31,8 @@ use crate::{InMemoryIndex, Manifest, MetadataResponse, ResolveError, VersionsRes
 pub struct ResolutionGraph {
     /// The underlying graph.
     pub(crate) petgraph: Graph<AnnotatedDist, Version, Directed>,
+    /// The range of supported Python versions.
+    pub(crate) requires_python: Option<VersionSpecifiers>,
     /// Any diagnostics that were encountered while building the graph.
     pub(crate) diagnostics: Vec<ResolutionDiagnostic>,
 }
@@ -39,9 +44,10 @@ impl ResolutionGraph {
         index: &InMemoryIndex,
         preferences: &Preferences,
         git: &GitResolver,
+        python: &PythonRequirement,
         resolution: Resolution,
     ) -> anyhow::Result<Self, ResolveError> {
-        // Collect all marker expressions from relevant pubgrub packages.
+        // Collect all marker expressions from relevant PubGrub packages.
         let mut markers: FxHashMap<(&PackageName, &Version, &Option<ExtraName>), MarkerTree> =
             FxHashMap::default();
         for (package, versions) in &resolution.packages {
@@ -267,8 +273,17 @@ impl ResolutionGraph {
             }
         }
 
+        // Extract the `Requires-Python` range, if provided.
+        // TODO(charlie): Infer the supported Python range from the `Requires-Python` of the
+        // included packages.
+        let requires_python = python
+            .target()
+            .and_then(RequiresPython::as_specifiers)
+            .cloned();
+
         Ok(Self {
             petgraph,
+            requires_python,
             diagnostics,
         })
     }
diff --git a/crates/uv-resolver/src/resolver/mod.rs b/crates/uv-resolver/src/resolver/mod.rs
index 145cd5802..b94995502 100644
--- a/crates/uv-resolver/src/resolver/mod.rs
+++ b/crates/uv-resolver/src/resolver/mod.rs
@@ -557,7 +557,13 @@ impl<InstalledPackages: InstalledPackagesProvider> ResolverState<InstalledPackag
         for resolution in resolutions {
             combined.union(resolution);
         }
-        ResolutionGraph::from_state(&self.index, &self.preferences, &self.git, combined)
+        ResolutionGraph::from_state(
+            &self.index,
+            &self.preferences,
+            &self.git,
+            &self.python_requirement,
+            combined,
+        )
     }
 
     /// Visit a [`PubGrubPackage`] prior to selection. This should be called on a [`PubGrubPackage`]
diff --git a/crates/uv-resolver/src/snapshots/uv_resolver__lock__tests__hash_optional_missing.snap b/crates/uv-resolver/src/snapshots/uv_resolver__lock__tests__hash_optional_missing.snap
index 60b21a96a..be90ce3be 100644
--- a/crates/uv-resolver/src/snapshots/uv_resolver__lock__tests__hash_optional_missing.snap
+++ b/crates/uv-resolver/src/snapshots/uv_resolver__lock__tests__hash_optional_missing.snap
@@ -73,6 +73,7 @@ Ok(
                 optional_dependencies: {},
             },
         ],
+        requires_python: None,
         by_id: {
             DistributionId {
                 name: PackageName(
diff --git a/crates/uv/tests/lock.rs b/crates/uv/tests/lock.rs
index 795670a57..a377d5016 100644
--- a/crates/uv/tests/lock.rs
+++ b/crates/uv/tests/lock.rs
@@ -1038,6 +1038,7 @@ fn lock_requires_python() -> Result<()> {
         assert_snapshot!(
             lock, @r###"
         version = 1
+        requires-python = ">=3.7"
 
         [[distribution]]
         name = "dataclasses"
@@ -1163,6 +1164,7 @@ fn lock_requires_python() -> Result<()> {
         assert_snapshot!(
             lock, @r###"
         version = 1
+        requires-python = ">=3.7.9, <4"
 
         [[distribution]]
         name = "attrs"