diff --git a/crates/uv-cli/src/lib.rs b/crates/uv-cli/src/lib.rs
index 3999e5144..b3e81906e 100644
--- a/crates/uv-cli/src/lib.rs
+++ b/crates/uv-cli/src/lib.rs
@@ -312,7 +312,7 @@ pub struct GlobalArgs {
     /// parent directories, or user configuration directories.
     ///
     /// This option is deprecated in favor of `--no-config`.
-    #[arg(global = true, long, hide = true)]
+    #[arg(global = true, long, hide = true, env = EnvVars::UV_ISOLATED, value_parser = clap::builder::BoolishValueParser::new())]
     pub isolated: bool,
 
     /// Show the resolved settings for the current command.
@@ -3169,7 +3169,7 @@ pub struct RunArgs {
     ///
     /// When used with `--with` or `--with-requirements`, the additional dependencies will still be
     /// layered in a second environment.
-    #[arg(long)]
+    #[arg(long, env = EnvVars::UV_ISOLATED, value_parser = clap::builder::BoolishValueParser::new())]
     pub isolated: bool,
 
     /// Prefer the active virtual environment over the project's virtual environment.
@@ -4476,7 +4476,7 @@ pub struct ToolRunArgs {
     pub overrides: Vec<Maybe<PathBuf>>,
 
     /// Run the tool in an isolated virtual environment, ignoring any already-installed tools.
-    #[arg(long)]
+    #[arg(long, env = EnvVars::UV_ISOLATED, value_parser = clap::builder::BoolishValueParser::new())]
     pub isolated: bool,
 
     /// Load environment variables from a `.env` file.
diff --git a/crates/uv-static/src/env_vars.rs b/crates/uv-static/src/env_vars.rs
index 42bb7c554..4a1011f29 100644
--- a/crates/uv-static/src/env_vars.rs
+++ b/crates/uv-static/src/env_vars.rs
@@ -136,6 +136,10 @@ impl EnvVars {
     /// directories.
     pub const UV_NO_CONFIG: &'static str = "UV_NO_CONFIG";
 
+    /// Equivalent to the `--isolated` command-line argument. If set, uv will avoid discovering
+    /// a `pyproject.toml` or `uv.toml` file.
+    pub const UV_ISOLATED: &'static str = "UV_ISOLATED";
+
     /// Equivalent to the `--exclude-newer` command-line argument. If set, uv will
     /// exclude distributions published after the specified date.
     pub const UV_EXCLUDE_NEWER: &'static str = "UV_EXCLUDE_NEWER";
diff --git a/docs/reference/cli.md b/docs/reference/cli.md
index 148cdebb4..b24860531 100644
--- a/docs/reference/cli.md
+++ b/docs/reference/cli.md
@@ -143,7 +143,7 @@ uv run [OPTIONS] [COMMAND]
 <p>Usually, the project environment is reused for performance. This option forces a fresh environment to be used for the project, enforcing strict isolation between dependencies and declaration of requirements.</p>
 <p>An editable installation is still used for the project.</p>
 <p>When used with <code>--with</code> or <code>--with-requirements</code>, the additional dependencies will still be layered in a second environment.</p>
-</dd><dt id="uv-run--keyring-provider"><a href="#uv-run--keyring-provider"><code>--keyring-provider</code></a> <i>keyring-provider</i></dt><dd><p>Attempt to use <code>keyring</code> for authentication for index URLs.</p>
+<p>May also be set with the <code>UV_ISOLATED</code> environment variable.</p></dd><dt id="uv-run--keyring-provider"><a href="#uv-run--keyring-provider"><code>--keyring-provider</code></a> <i>keyring-provider</i></dt><dd><p>Attempt to use <code>keyring</code> for authentication for index URLs.</p>
 <p>At present, only <code>--keyring-provider subprocess</code> is supported, which configures uv to use the <code>keyring</code> CLI to handle authentication.</p>
 <p>Defaults to <code>disabled</code>.</p>
 <p>May also be set with the <code>UV_KEYRING_PROVIDER</code> environment variable.</p><p>Possible values:</p>
@@ -2034,7 +2034,7 @@ uv tool run [OPTIONS] [COMMAND]
 <p>Accepts either a repository compliant with PEP 503 (the simple repository API), or a local directory laid out in the same format.</p>
 <p>The index given by this flag is given lower priority than all other indexes specified via the <code>--extra-index-url</code> flag.</p>
 <p>May also be set with the <code>UV_INDEX_URL</code> environment variable.</p></dd><dt id="uv-tool-run--isolated"><a href="#uv-tool-run--isolated"><code>--isolated</code></a></dt><dd><p>Run the tool in an isolated virtual environment, ignoring any already-installed tools</p>
-</dd><dt id="uv-tool-run--keyring-provider"><a href="#uv-tool-run--keyring-provider"><code>--keyring-provider</code></a> <i>keyring-provider</i></dt><dd><p>Attempt to use <code>keyring</code> for authentication for index URLs.</p>
+<p>May also be set with the <code>UV_ISOLATED</code> environment variable.</p></dd><dt id="uv-tool-run--keyring-provider"><a href="#uv-tool-run--keyring-provider"><code>--keyring-provider</code></a> <i>keyring-provider</i></dt><dd><p>Attempt to use <code>keyring</code> for authentication for index URLs.</p>
 <p>At present, only <code>--keyring-provider subprocess</code> is supported, which configures uv to use the <code>keyring</code> CLI to handle authentication.</p>
 <p>Defaults to <code>disabled</code>.</p>
 <p>May also be set with the <code>UV_KEYRING_PROVIDER</code> environment variable.</p><p>Possible values:</p>
diff --git a/docs/reference/environment.md b/docs/reference/environment.md
index 2a5131048..f0a73fce3 100644
--- a/docs/reference/environment.md
+++ b/docs/reference/environment.md
@@ -191,6 +191,11 @@ in lieu of the default GitHub URL.
 The directory in which to install uv using the standalone installer and `self update` feature.
 Defaults to `~/.local/bin`.
 
+### `UV_ISOLATED`
+
+Equivalent to the `--isolated` command-line argument. If set, uv will avoid discovering
+a `pyproject.toml` or `uv.toml` file.
+
 ### `UV_KEYRING_PROVIDER`
 
 Equivalent to the `--keyring-provider` command-line argument. If set, uv